Journal: Chinese Medicine

Article Title: Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

doi: 10.1186/s13020-024-00881-6

Figure Lengend Snippet: S.C targets regulation of HMOX1 expression Volcano plots for differentially expressed genes in PC3 a and DU145 b cells receiving 1.0 μM S. C treatment for 12 h or not. c Intersections of differentially expressed genes with in PC3 and DU145 cells. d List of differentially expressed genes in DU145 and PC3 cells. mRNA expression level of HMOX1 in PC3 and DU145 cells receiving S.C treatment or not ( e , f ). Protein level of HMOX1 in PC3 and DU145 cells receiving S.C treatment or not ( g , h )

Article Snippet: HMOX1 knockdown vectors (pLKO.1- HMOX1-a/b/c ) were synthesized by Synbio-Technologies.

Techniques: Expressing

Journal: Chinese Medicine

Article Title: Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

doi: 10.1186/s13020-024-00881-6

Figure Lengend Snippet: S.C inducing HMOX-1 up-regulation contributes to ferroptosis in prostate cancer cell. mRNA and protein levels of HMOX1 measured by qRT-PCR and western blot in PC3 HMOX1 knockdown a , c and DU145 HMOX1 knockdown cells b , d . DCFH-DA probe was used to detected ROS levels in PC3 HMOX1 knockdown e and DU145 HMOX1 knockdown f cells with S.C treatment or not. Mito-FerroGreen probe was used to detected iron levels in PC3 HMOX1 knockdown g and DU145 HMOX1 knockdown (h) cells. i , j Quantification of the MDA levels in HMOX1 knockdown cells. GPX4 and SLC7A11 protein level in PC3 HMOX1 knockdown k and DU145 HMOX1 knockdown l cells

Article Snippet: HMOX1 knockdown vectors (pLKO.1- HMOX1-a/b/c ) were synthesized by Synbio-Technologies.

Techniques: Quantitative RT-PCR, Western Blot, Knockdown

Journal: Chinese Medicine

Article Title: Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

doi: 10.1186/s13020-024-00881-6

Figure Lengend Snippet: S.C induces HMOX1 up-regulation depending on decreasing the stability of BACH1 protein. Western blotting analysis of PERK, STAT3, NRF2, BACH1, KEAP1, and HIF-1α levels in PC3 a and DU145 b cells receiving S.C (0, 0.5, 1.0 μM) treatment for 48 h. Immunoblotting analysis of BACH1 in nuclear and cytoplasm of S.C (0, 0.5, 1.0 μM)-treated PC3 c and DU145 d cells. e mRNA expression level of BACH1 in S.C-treated PC3 and DU145 cells. f – g Cycloheximide (CHX) chase assay in S.C-treated DU145 cells. h BACH1 protein levels were calculated by quantification of BACH1protein levels. Immunoblotting analysis of USP47 in S.C (0, 0.5, 1.0 μM)-treated PC3 i and DU145 j cells. Immunoblotting analysis of BACH1 and HMOX1 in S.C (0, 1.0 μM)-treated PC3 k and DU145 l cells transiently expressing empty vector or BACH1 vector

Article Snippet: HMOX1 knockdown vectors (pLKO.1- HMOX1-a/b/c ) were synthesized by Synbio-Technologies.

Techniques: Western Blot, Expressing, Plasmid Preparation

Journal: Chinese Medicine

Article Title: Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

doi: 10.1186/s13020-024-00881-6

Figure Lengend Snippet: ROS is responsible for BACH1 decreasing in S.C inducing ferroptosis. Cells were pretreated with vehicle or 5 mM NAC for 1 h before treatment with 0.5 μM S.C in PC3 a , b , g , h or DU145 c , d , i , j cells. After 24 h treatment with vehicle or S.C in the presence of NAC, cells were stained with DCFH-DA or Mito-FerroGreen for flow cytometry assay. After 48 h treatment with vehicle or S.C in the presence of NAC, cells were used to analyze the contents of GSH, MDA in PC3 e , k and DU145 cells f , l . After 48 h treatment, GPX4, SLC7A11, HMOX1, BACH1 and USP47 levels were analyzed by Immunoblotting in PC3 m and DU145 cells n

Article Snippet: HMOX1 knockdown vectors (pLKO.1- HMOX1-a/b/c ) were synthesized by Synbio-Technologies.

Techniques: Staining, Flow Cytometry, Western Blot

Journal: Chinese Medicine

Article Title: Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

doi: 10.1186/s13020-024-00881-6

Figure Lengend Snippet: S.C treatment inhibits cancer progression in DU145 xenograft model. DU145 cells were subcutaneously injected into the flank of NCG nude mice. 10 days after inoculation, the mice were treated with S.C (2.5, 5.0 mg/kg body weight) via the intraperitoneal (i.p) route every 2 days. a 2 weeks after injection, tumor tissues were excised and photographed. The excised tumor weight b and volume c were calculated. d Body weight was measured every 2 days after administration of S.C. e Tumor volume was calculated every 2 days after administration of S.C. f Representative images of H&E and immunohistochemical staining for Ki67 and HMOX1 g protein levels of GPX4, SLC7A11, caspase3, HMOX1, USP47 and BACH1 measured by western blot

Article Snippet: HMOX1 knockdown vectors (pLKO.1- HMOX1-a/b/c ) were synthesized by Synbio-Technologies.

Techniques: Injection, Immunohistochemical staining, Staining, Western Blot